在DataGridView属性里面添加dgvOne_CellValidating事件，然后根据需要以后。

   private void dgvOne_CellValidating(object sender, DataGridViewCellValidatingEventArgs e)

    {

        //可编辑的列2、3、4、5、6（实际显示的列减1）列需要输入0~9的自然数.

        if ( e.ColumnIndex == 2 || e.ColumnIndex == 3 || e.ColumnIndex == 4 || e.ColumnIndex == 5 || e.ColumnIndex == 6)

        {

            int outDb = 0;

            if (int.TryParse(e.FormattedValue.ToString(), out outDb))

            {

                e.Cancel = false;

            }

            else

            {

                e.Cancel = true;//数据格式不正确则还原

                MessageBox.Show("请输入0~9的自然数！", "提交提示", MessageBoxButtons.OK, MessageBoxIcon.Information);

                dgvOne.CancelEdit();

            }

        }

      //第二列验证时间格式

        if (e.ColumnIndex == 1)

        {

            DateTime  outDb =DateTime.Now;

            if (DateTime.TryParse(e.FormattedValue.ToString(), out outDb))

            {

                e.Cancel = false;

            }

            else

            {

                e.Cancel = true;//数据格式不正确则还原

                MessageBox.Show("输入日期格式不正确，请重新输入！", "提交提示", MessageBoxButtons.OK, MessageBoxIcon.Information);

                dgvOne.CancelEdit();

            }

        }

            //第一列验证正整数

        if (e.ColumnIndex == 0)

        {

            Regex notWholePattern = new Regex(@"^[0-9]\d*$");

            if (notWholePattern.IsMatch(e.FormattedValue.ToString(), 0))

            {

                e.Cancel = false;

            }

            else

            {

                e.Cancel = true;//数据格式不正确则还原

                MessageBox.Show("输入序号的格式不正确，请重新输入正整数！", "提交提示", MessageBoxButtons.OK, MessageBoxIcon.Information);

                dgvTwo.CancelEdit();

            }

        }

       //长度验证

        if (e.ColumnIndex == 6 || e.ColumnIndex == 7)

        {

            string str = e.FormattedValue.ToString();

            int leng = str.Length;

            if (leng > 1)

            {

                e.Cancel = true;//数据格式不正确则还原

                MessageBox.Show("只能输入一位0~9的自然数，请重新输入！", "提交提示", MessageBoxButtons.OK, MessageBoxIcon.Information);

                dgvTwo.CancelEdit();

            }

            else

            {

                e.Cancel = false;

            }

        }

    }